The Holt Presbyterian Church was organized on April 5, 1865 by seventeen Scotch Presbyterians who wanted a church they could attend according to their customs. James Thorburn, Sr., Casper Lott, and Church Wilbur were selected as the first Elders and were ordained. At the beginning the church met at the Delhi Center school building. On October 21, 1867, the church purchased a building site for forty-two dollars, the building was completed in 1869. It was an oak frame structure with hand hewn beams and a capacity of 200, at a cost of two thousand dollars. The building, dedicated on October 3, 1869, had a balcony and was heated by wood stoves.

From the beginning of the church through 1900, nineteen pastors ministered the church. The congregation became responsive to building a new church building in 1900. $2,671 was raised and a cornerstone for the new church building was laid in July of 1900. The new building was dedicated in February of 1901.

The original thirty year charter of the church expired in January of 1912 and the congregation re-organized and incorporated as the “First Presbyterian Church of Holt, Michigan.”

In 1955, land was purchased at the northwest corner of Holt and Aurelius Roads for sixteen thousand dollars. The church had been saving to build a new building since 1951. In 1963, the church broke ground on their new building and demolished their building that was built in 1900. The corner stone for the new building was laid in June 1963. The first services were held in March of 1964 and the building was dedicated in October 1964. The church building at Holt and Aurelius Roads still functions as the home of the First Presbyterian Church of Holt.

The church celebrated their 150th anniversary in 2014-15. April 5, 2015 marked 150 years since the establishment of the Presbyterian Church in Holt. The cornerstone and bell from the 1900-1964 church building are incorporated into a monument on the lawn of the current building.